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anti-rabbit alsin (Millipore)

Name: anti-rabbit alsin
Brand: Millipore
Rating: 90 (1 reviews)

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Millipore anti-rabbit alsin
BAC GFP-Rab5 cells were seeded on glass coverslips, fixed, and immunostained with antibodies against <t>Rabenosyn-5</t> ( A ), EEA1 ( B ), APPL1 ( C ), and APPL2 ( D ). Inset regions are shown. Scale bars, 10 μm.

BAC GFP-Rab5 cells were seeded on glass coverslips, fixed, and immunostained with antibodies against <t>Rabenosyn-5</t> ( A ), EEA1 ( B ), APPL1 ( C ), and APPL2 ( D ). Inset regions are shown. Scale bars, 10 μm.

Anti Rabbit Alsin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-rabbit alsin/product/Millipore
Average 90 stars, based on 1 article reviews

anti-rabbit alsin - by Bioz Stars, 2026-02

90/100 stars

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1) Product Images from "Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria"

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

Journal: eLife

doi: 10.7554/eLife.32282

BAC GFP-Rab5 cells were seeded on glass coverslips, fixed, and immunostained with antibodies against Rabenosyn-5 ( A ), EEA1 ( B ), APPL1 ( C ), and APPL2 ( D ). Inset regions are shown. Scale bars, 10 μm.

Figure Legend Snippet: BAC GFP-Rab5 cells were seeded on glass coverslips, fixed, and immunostained with antibodies against Rabenosyn-5 ( A ), EEA1 ( B ), APPL1 ( C ), and APPL2 ( D ). Inset regions are shown. Scale bars, 10 μm.

Techniques Used:

$BAC GFP-Rab5 cells seeded on gridded dishes were labeled with Mito-Red and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with antibodies against ZFYVE20 and EEA1 ( A ), or APPL1 and APPL2 ( B ). Inset regions are shown. Scale bars, 10 μm. ( C ) Colocalization analysis from untreated and laser-treated cells in ( A ) and ( B ), n = 3. p Values based on two-tailed t-tests. ( D ) Subcellular fractionation was performed in HeLa cells treated with either PBS (control) or 250 μM H 2 O 2 at 37°C for 2 hr. Protein samples from purified mitochondria (Mito) and cytosolic ( C ) fractions were loaded onto SDS-PAGE and imunoblotted with antibodies against Rab5, EEA1, Rabenosyn-5, APPL1, APPL2, and TOM20. 10.7554/eLife.32282.041 Figure 9—source data 1. Numerical data corresponding to the bar graphs presented in .$
Figure Legend Snippet: BAC GFP-Rab5 cells seeded on gridded dishes were labeled with Mito-Red and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with antibodies against ZFYVE20 and EEA1 ( A ), or APPL1 and APPL2 ( B ). Inset regions are shown. Scale bars, 10 μm. ( C ) Colocalization analysis from untreated and laser-treated cells in ( A ) and ( B ), n = 3. p Values based on two-tailed t-tests. ( D ) Subcellular fractionation was performed in HeLa cells treated with either PBS (control) or 250 μM H 2 O 2 at 37°C for 2 hr. Protein samples from purified mitochondria (Mito) and cytosolic ( C ) fractions were loaded onto SDS-PAGE and imunoblotted with antibodies against Rab5, EEA1, Rabenosyn-5, APPL1, APPL2, and TOM20. 10.7554/eLife.32282.041 Figure 9—source data 1. Numerical data corresponding to the bar graphs presented in .

Techniques Used: Labeling, Two Tailed Test, Fractionation, Purification, SDS Page

In the normal condition, mitochondria (Mito, red) are elongated and tubular (left). Rab5 (green) are localized on early endosomes (EE) to assemble the Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, some EE make transient contacts with mitochondria. During oxidative stress (e.g. laser- or chemically-induced), mitochondria undergo MOMP and a dramatic morphological transformation into rounded and swollen structures (right). The release of the apoptotic factor, cytochrome c, from mitochondria into the cytosol is associated with a re-localization event of Rab5 from EE to mitochondria via a cytosolic intermediate, accompanied by an increase in EE-mitochondria MCS. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), which leads to a selective recruitment of Rabenosyn-5 (light green). This signaling cascade on mitochondria is a reversible process that regulates the apoptotic program (e.g. cytochrome c release and caspase activation) and thus, promotes overall cell survival.

Figure Legend Snippet: In the normal condition, mitochondria (Mito, red) are elongated and tubular (left). Rab5 (green) are localized on early endosomes (EE) to assemble the Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, some EE make transient contacts with mitochondria. During oxidative stress (e.g. laser- or chemically-induced), mitochondria undergo MOMP and a dramatic morphological transformation into rounded and swollen structures (right). The release of the apoptotic factor, cytochrome c, from mitochondria into the cytosol is associated with a re-localization event of Rab5 from EE to mitochondria via a cytosolic intermediate, accompanied by an increase in EE-mitochondria MCS. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), which leads to a selective recruitment of Rabenosyn-5 (light green). This signaling cascade on mitochondria is a reversible process that regulates the apoptotic program (e.g. cytochrome c release and caspase activation) and thus, promotes overall cell survival.

Techniques Used: Transformation Assay, Activation Assay

Image Search Results

Figure 10. Localization of Rabex-5 and Alsin upon mitochondrial stress. BAC GFP-Rab5 cells seeded on gridded dishes were labeled with 100 nM Mito-Red and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with antibody against Rabex-5 (A) or Alsin (B). Inset regions are shown. Scale bars, 10 mm. (C) Colocalization analysis from untreated and laser-treated cells in (A) and (B), n = 3. p-Values based on two-tailed t-tests. DOI: https://doi.org/10.7554/eLife.32282.042 The following source data and figure supplements are available for figure 10:

Journal: eLife

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.7554/elife.32282

Figure Lengend Snippet: Figure 10. Localization of Rabex-5 and Alsin upon mitochondrial stress. BAC GFP-Rab5 cells seeded on gridded dishes were labeled with 100 nM Mito-Red and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with antibody against Rabex-5 (A) or Alsin (B). Inset regions are shown. Scale bars, 10 mm. (C) Colocalization analysis from untreated and laser-treated cells in (A) and (B), n = 3. p-Values based on two-tailed t-tests. DOI: https://doi.org/10.7554/eLife.32282.042 The following source data and figure supplements are available for figure 10:

Article Snippet: Cells were immunostained with the corresponding primary antibodies: anti-rabbit Rabenosyn-5/ ZFYVE20 (Sigma Aldrich: HPA044878), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse APPL2 (home-made), anti-mouse Rab5 (BD Biosciences: 610724), anti-mouse cytochrome c (Abcam: ab6311), and anti-rabbit Alsin (Novus Biological: NBP2-14284) antibodies.

Techniques: Labeling, Two Tailed Test

$Figure 11. Alsin is required for Rab5 recruitment and regulates cytochrome c release. (A) Flow chart depicting the different stages and time (in days) from induced-pluripotent stem cells (iPSC), to neuroprogenitor cells (NPC), and to mature spinal motor neurons (sMN). The small molecules and compounds used at different stages are shown and color-coded. (B) WT and Alsin-/- cells were challenged with either PBS (Ctrl) or 100 mM H2O2 at 37˚C for 1 hr. Cells were fixed and immunostained with Rab5 and TOM20 antibodies. Inset images show are shown. Scale bars, 10 mm. (C) Subcellular fractionation of mitochondrial (Mito) and cytosolic (C) fractions from WT and Alsin-/- iPSC- sMN challenged with either PBS or 100 mM H2O2 at 37˚C for 1 hr. Protein samples were loaded onto SDS-PAGE and imunoblotted with antibodies against Rab5, tubulin and TOM20. (D) Cytosolic fractions were prepared from WT and Alsin-/- iPSC-sMN challenged with 100 mM H2O2 at 37˚C for 1 hr. Densitometric quantification of cytosolic cytochrome c were collected from three independent experiments. Y-axis corresponds to normalized ratio intensity of cytochrome c to tubulin. Error bars represent SEMs. p-Values based on two-tailed t-tests. DOI: https://doi.org/10.7554/eLife.32282.047 The following source data and figure supplement are available for figure 11:$

Journal: eLife

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.7554/elife.32282

Figure Lengend Snippet: Figure 11. Alsin is required for Rab5 recruitment and regulates cytochrome c release. (A) Flow chart depicting the different stages and time (in days) from induced-pluripotent stem cells (iPSC), to neuroprogenitor cells (NPC), and to mature spinal motor neurons (sMN). The small molecules and compounds used at different stages are shown and color-coded. (B) WT and Alsin-/- cells were challenged with either PBS (Ctrl) or 100 mM H2O2 at 37˚C for 1 hr. Cells were fixed and immunostained with Rab5 and TOM20 antibodies. Inset images show are shown. Scale bars, 10 mm. (C) Subcellular fractionation of mitochondrial (Mito) and cytosolic (C) fractions from WT and Alsin-/- iPSC- sMN challenged with either PBS or 100 mM H2O2 at 37˚C for 1 hr. Protein samples were loaded onto SDS-PAGE and imunoblotted with antibodies against Rab5, tubulin and TOM20. (D) Cytosolic fractions were prepared from WT and Alsin-/- iPSC-sMN challenged with 100 mM H2O2 at 37˚C for 1 hr. Densitometric quantification of cytosolic cytochrome c were collected from three independent experiments. Y-axis corresponds to normalized ratio intensity of cytochrome c to tubulin. Error bars represent SEMs. p-Values based on two-tailed t-tests. DOI: https://doi.org/10.7554/eLife.32282.047 The following source data and figure supplement are available for figure 11:

Techniques: Fractionation, SDS Page, Two Tailed Test

Figure 12. Schematic model depicting the role of Rab5-Alsin-mitochondria during oxidative stress. In the normal condition, mitochondria (Mito, red) are elongated and tubular (left). Rab5 (green) are localized on early endosomes (EE) to assemble the Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, some EE make transient contacts with mitochondria. During oxidative stress (e.g. laser- or chemically- induced), mitochondria undergo MOMP and a dramatic morphological transformation into rounded and swollen structures (right). The release of the apoptotic factor, cytochrome c, from mitochondria into the cytosol is associated with a re-localization event of Rab5 from EE to mitochondria via a cytosolic intermediate, accompanied by an increase in EE-mitochondria MCS. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), which leads to a selective recruitment of Rabenosyn-5 (light green). This signaling cascade on mitochondria is a reversible process that regulates the apoptotic program (e.g. cytochrome c release and caspase activation) and thus, promotes overall cell survival. DOI: https://doi.org/10.7554/eLife.32282.050

Journal: eLife

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.7554/elife.32282

Figure Lengend Snippet: Figure 12. Schematic model depicting the role of Rab5-Alsin-mitochondria during oxidative stress. In the normal condition, mitochondria (Mito, red) are elongated and tubular (left). Rab5 (green) are localized on early endosomes (EE) to assemble the Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, some EE make transient contacts with mitochondria. During oxidative stress (e.g. laser- or chemically- induced), mitochondria undergo MOMP and a dramatic morphological transformation into rounded and swollen structures (right). The release of the apoptotic factor, cytochrome c, from mitochondria into the cytosol is associated with a re-localization event of Rab5 from EE to mitochondria via a cytosolic intermediate, accompanied by an increase in EE-mitochondria MCS. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), which leads to a selective recruitment of Rabenosyn-5 (light green). This signaling cascade on mitochondria is a reversible process that regulates the apoptotic program (e.g. cytochrome c release and caspase activation) and thus, promotes overall cell survival. DOI: https://doi.org/10.7554/eLife.32282.050

Techniques: Membrane, Transformation Assay, Activation Assay

Journal: eLife

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.7554/eLife.32282

Figure Lengend Snippet: BAC GFP-Rab5 cells were seeded on glass coverslips, fixed, and immunostained with antibodies against Rabenosyn-5 ( A ), EEA1 ( B ), APPL1 ( C ), and APPL2 ( D ). Inset regions are shown. Scale bars, 10 μm.

Article Snippet: The following antibodies were used in western blot: anti-mouse cytochrome c (Abcam: ab13575), anti-rabbit gamma tubulin (Sigma-Aldrich: T6557), anti-rabbit Rabenosyn-5/ZFYVE20, anti-rabbit Alsin (Sigma Aldrich: SAB4200137), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse APPL2 (home-made), anti-mouse GAPDH (Sigma Aldrich: G8795), anti-mouse gamma tubulin (Sigma Aldrich: T6557), and anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415).

Techniques:

Journal: eLife

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.7554/eLife.32282

Figure Lengend Snippet: BAC GFP-Rab5 cells seeded on gridded dishes were labeled with Mito-Red and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with antibodies against ZFYVE20 and EEA1 ( A ), or APPL1 and APPL2 ( B ). Inset regions are shown. Scale bars, 10 μm. ( C ) Colocalization analysis from untreated and laser-treated cells in ( A ) and ( B ), n = 3. p Values based on two-tailed t-tests. ( D ) Subcellular fractionation was performed in HeLa cells treated with either PBS (control) or 250 μM H 2 O 2 at 37°C for 2 hr. Protein samples from purified mitochondria (Mito) and cytosolic ( C ) fractions were loaded onto SDS-PAGE and imunoblotted with antibodies against Rab5, EEA1, Rabenosyn-5, APPL1, APPL2, and TOM20. 10.7554/eLife.32282.041 Figure 9—source data 1. Numerical data corresponding to the bar graphs presented in .

Techniques: Labeling, Two Tailed Test, Fractionation, Purification, SDS Page

Journal: eLife

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.7554/eLife.32282

Figure Lengend Snippet: In the normal condition, mitochondria (Mito, red) are elongated and tubular (left). Rab5 (green) are localized on early endosomes (EE) to assemble the Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, some EE make transient contacts with mitochondria. During oxidative stress (e.g. laser- or chemically-induced), mitochondria undergo MOMP and a dramatic morphological transformation into rounded and swollen structures (right). The release of the apoptotic factor, cytochrome c, from mitochondria into the cytosol is associated with a re-localization event of Rab5 from EE to mitochondria via a cytosolic intermediate, accompanied by an increase in EE-mitochondria MCS. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), which leads to a selective recruitment of Rabenosyn-5 (light green). This signaling cascade on mitochondria is a reversible process that regulates the apoptotic program (e.g. cytochrome c release and caspase activation) and thus, promotes overall cell survival.

Techniques: Transformation Assay, Activation Assay

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